In the past, methods for detecting Listeria Monocytogenes from foods and for confirmation, was through prolonged incubation and biochemical testing. These methods requires up to four to five days to obtain results.
However, in a current study, it has shown that a simple and rapid proteomics-based MALDI-TOF MS approach was developed to detect L. monocytogenes directly from selective enrichment broths. Milk samples spiked with single or multiple species were incubated in a selective enrichment broth for 24 hours, followed by a 6 hours secondary enrichment. Low amounts of colony-forming unit (cfu) of Listeria monocytogenes were detected in the first 30 hours.
They have also carried out the same procedures for solid foods, namely, chicken pâté, cantaloupe and Camembert cheese were used. Detection was achieved by using this method. This new detection method saves time and money for a more direct identification of Listeria monocytogenes.
Listeria monocytogenes have the potential to cause major illnesses and fatalities in humans. Hence, continual development of new methods for detection is essential. Therefore, this method presents itself as a useful tool to detecting the major food-borne pathogen, Listeria monocytogenes.
References
S, J., D, S., M, B. & EA, P., 2014. Detection of Listeria monocytogenes from selective enrichment broth using MALDI-TOF Mass Spectrometry. [Online]
Available at: https://www.ncbi.nlm.nih.gov/pubmed/24080423
[Accessed 25 7 2018].
Healthy fatty acids can work as medicine to neutralize listeria. Professor Birgitte Kallipolitis had proved that Omega-3 fatty acids can neutralise listeria in 30 minutes. However, too low amount of fatty acid – Omega-3 will not kill the bacteria.
Radagest Pet Food Inc. of Portland, OR, has recently recalled their raw cat food back due to the possibility of having listeria monocytogenes. It was recalled along with the likelihood of the presence of Escherichia Coli, which was also detected during the testing.
Listeria 